美國Applied Biosystems? 7500實(shí)時(shí)熒光定量PCR系統(tǒng)
美國Applied Biosystems? 7500實(shí)時(shí)熒光定量PCR系統(tǒng) | 時(shí)間: December-25 14:54:34 | 分類:其它儀器
凌儀生物專業(yè)提供高速離心機(jī)，美國Bio-rad伯樂電泳儀，德國eppendorf艾本德移液器，PCR基因擴(kuò)增儀，雅培原位雜交儀，Thermo熱電離心機(jī)等實(shí)驗(yàn)室儀器設(shè)備，要省錢，別錯(cuò)過哦。 
7500實(shí)時(shí)熒光定量PCR系統(tǒng)描述
The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This system is a sophisticated platform for users who require extended capabilities and maximum versatility. The 3rd generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.
• Powerful, five-color platform is calibrated for the broadest range of dyes available: FAM™⁄SYBR® Green I, VIC®⁄JOE™, NED™⁄ TAMRA™⁄ Cy3™, ROX™⁄Texas Red®, and Cy5™ dyes
• Specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets
• Advanced multi-componenting algorithm minimizes spectral crosstalk – superior for multiplexing
• User-friendly software includes plate set-up wizards, multi-plate data viewing capabilities, and advanced analysis tools to make data processing simple and straightforward
NEW - 7500 Software v2.0
Now the easy-to-use StepOne software is available for both the 7500 and 7500 Fast systems with the 7500 Software v2.0 upgrade. The 7500 Software v2.0 incorporates your favorite StepOne Software features, such as a variety of plate setup wizards, standard curve dilution and master mix recipe calculators, QC flags, data filters, and email notification when a run is finished. The 7500 Software v2.0 also includes an enhanced Gene Expression Study package and has a variety of new melting curve protocol options, including multiple peak detection, step and hold temperature control, and customizable ramp rates.
The NEW Gene Expression Study package accommodates large studies better than any other instrument software package:
• Import an unlimited number of Comparative CT (relative quantitation) files to one study
• Group samples and view data both by technical replicate group and biological replicate group
• Use any gene(s) as an endogenous control, including averaging multiple controls together
• Enter known efficiency values to be factored into the RQ results
21 CFR Part 11 Module available
The SDS v1.4 21CFRp11 Module is a powerful tool for assisting with 21CFRp11 compliance, while still offering the flexibility of user-customizable configuration settings.
• Individual user log-ins can be added for up to four user groups, each group with designated permission settings
• User-customizable permission settings include fourteen system activities, e-signature authority designation, and additional security settings to give you maximum control over your compliance efforts
• Audit trails can be enabled or disabled depending on your traceability needs
• A selection of e-signatures is available to ease e-signatures into your workflow
Supports Many Applications
Applications include gene expression analysis, pathogen quantitation, SNP genotyping, isothermal and +⁄– assays utilizing internal positive controls. To facilitate many of these applications, Applied Biosystems® provides preformulated, ready-to-use, quality-tested, TaqMan® assays for use with the 7500 system. Now you can reduce your assay optimization efforts.
Upgrade to High-Speed Thermal Cycling
An optional upgrade to the 7500 Fast System is available. This 7500 Fast System uses our master mix formulations and enables you to shorten your real-time PCR runs to as little as 30 minutes.
The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This system is a sophisticated platform for users who require extended capabilities and maximum versatility. The 3rd generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.
• Powerful, five-color platform is calibrated for the broadest range of dyes available: FAM™⁄SYBR® Green I, VIC®⁄JOE™, NED™⁄ TAMRA™⁄ Cy3™, ROX™⁄Texas Red®, and Cy5™ dyes
• Specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets
• Advanced multi-componenting algorithm minimizes spectral crosstalk – superior for multiplexing
• User-friendly software includes plate set-up wizards, multi-plate data viewing capabilities, and advanced analysis tools to make data processing simple and straightforward
NEW - 7500 Software v2.0
Now the easy-to-use StepOne software is available for both the 7500 and 7500 Fast systems with the 7500 Software v2.0 upgrade. The 7500 Software v2.0 incorporates your favorite StepOne Software features, such as a variety of plate setup wizards, standard curve dilution and master mix recipe calculators, QC flags, data filters, and email notification when a run is finished. The 7500 Software v2.0 also includes an enhanced Gene Expression Study package and has a variety of new melting curve protocol options, including multiple peak detection, step and hold temperature control, and customizable ramp rates.
The NEW Gene Expression Study package accommodates large studies better than any other instrument software package:
• Import an unlimited number of Comparative CT (relative quantitation) files to one study
• Group samples and view data both by technical replicate group and biological replicate group
• Use any gene(s) as an endogenous control, including averaging multiple controls together
• Enter known efficiency values to be factored into the RQ results
21 CFR Part 11 Module available
The SDS v1.4 21CFRp11 Module is a powerful tool for assisting with 21CFRp11 compliance, while still offering the flexibility of user-customizable configuration settings.
• Individual user log-ins can be added for up to four user groups, each group with designated permission settings
• User-customizable permission settings include fourteen system activities, e-signature authority designation, and additional security settings to give you maximum control over your compliance efforts
• Audit trails can be enabled or disabled depending on your traceability needs
• A selection of e-signatures is available to ease e-signatures into your workflow
Supports Many Applications
Applications include gene expression analysis, pathogen quantitation, SNP genotyping, isothermal and +⁄– assays utilizing internal positive controls. To facilitate many of these applications, Applied Biosystems® provides preformulated, ready-to-use, quality-tested, TaqMan® assays for use with the 7500 system. Now you can reduce your assay optimization efforts.
Upgrade to High-Speed Thermal Cycling
An optional upgrade to the 7500 Fast System is available. This 7500 Fast System uses our master mix formulations and enables you to shorten your real-time PCR runs to as little as 30 minutes.
? 功能強(qiáng)大的五色平臺(tái)已經(jīng)過校準(zhǔn)，支持較多種類的染料：FAM™/SYBR® Green I、VIC®/JOE™、ED™/TAMRA™/Cy®3、ROX™/Texas Red® 和 Cy®5 染料Applied Biosystems® 7500 實(shí)時(shí)熒光定量 PCR 系統(tǒng)為要求較大的染料功能多樣性的實(shí)驗(yàn)室提供了一種可靠的平臺(tái)。該平臺(tái)具有靈敏的光學(xué)系統(tǒng)，適用于寬廣光譜范圍內(nèi)的多種熒光染料?？勺兊募ぐl(fā)波長可提高長波長 (紅色) 染料的檢測(cè)靈敏度。
7500 軟件
7500 軟件融合了多種易于使用的特性，如各種反應(yīng)板設(shè)置向?qū)?、?biāo)準(zhǔn)曲線稀釋和預(yù)混液配方計(jì)算器、QC標(biāo)記、數(shù)據(jù)過濾器，并可在運(yùn)行完成時(shí)通過電子郵件通知。7500 軟件還包括功能強(qiáng)大的基因表達(dá)研究軟件包，并提供各種熔解曲線實(shí)驗(yàn)方案選項(xiàng)，包括多峰檢測(cè)、步進(jìn)式溫度控制和可定制的變溫速率?？商峁┳裱绹?lián)邦法規(guī)第 21 章第 11 節(jié) (21 CFR Part 11) 的模塊SDS v1.4 21CFR Part 11 模塊是一款功能強(qiáng)大的工具，它遵循美國聯(lián)邦法規(guī)第 21 章第 11 節(jié)的要求，同時(shí)還可提供用戶可定制的配置設(shè)置靈活性。
支持多種應(yīng)用
其應(yīng)用領(lǐng)域包括基因表達(dá)分析、病原體定量、SNP 基因分型、拷貝數(shù)變異、恒溫和采用內(nèi)部陽性對(duì)照的 +/–Assay。為方便上述應(yīng)用領(lǐng)域，Life Technologies 提供了預(yù)先配制、已經(jīng)過質(zhì)量測(cè)試的即用型 TaqMan® Assay，可與 7500 系統(tǒng)配合使用?，F(xiàn)在您即可節(jié)省在分析優(yōu)化方面的精力。
升級(jí)至 7500 Fast 系統(tǒng)
您可以選擇升級(jí)至 7500 Fast 系統(tǒng)。該 7500 Fast 系統(tǒng)采用了我們的預(yù)混液配方，使您可以將實(shí)時(shí)熒光定量 PCR 程序縮短至 30 分鐘。
7500 系統(tǒng)包括：
? 可選擇 Dell® 商用系列筆記本或配有平板顯示器的臺(tái)式電腦
? 化學(xué)安裝試劑盒包括染料校正反應(yīng)板、RNA酶 P 儀器驗(yàn)證反應(yīng)板和應(yīng)用特異的 starter kit
? 儀器操作和分析軟件，以及 Primer Express®引物和探針設(shè)計(jì)軟件
? 包括入門指南在內(nèi)的豐富的文件集，適用于各種主要的實(shí)時(shí)熒光定量 PCR 應(yīng)用領(lǐng)域
? 由 Life Technologies 服務(wù)工程師提供的安裝服務(wù)
7500 軟件
7500 軟件融合了多種易于使用的特性，如各種反應(yīng)板設(shè)置向?qū)?、?biāo)準(zhǔn)曲線稀釋和預(yù)混液配方計(jì)算器、QC標(biāo)記、數(shù)據(jù)過濾器，并可在運(yùn)行完成時(shí)通過電子郵件通知。7500 軟件還包括功能強(qiáng)大的基因表達(dá)研究軟件包，并提供各種熔解曲線實(shí)驗(yàn)方案選項(xiàng)，包括多峰檢測(cè)、步進(jìn)式溫度控制和可定制的變溫速率?？商峁┳裱绹?lián)邦法規(guī)第 21 章第 11 節(jié) (21 CFR Part 11) 的模塊SDS v1.4 21CFR Part 11 模塊是一款功能強(qiáng)大的工具，它遵循美國聯(lián)邦法規(guī)第 21 章第 11 節(jié)的要求，同時(shí)還可提供用戶可定制的配置設(shè)置靈活性。
支持多種應(yīng)用
其應(yīng)用領(lǐng)域包括基因表達(dá)分析、病原體定量、SNP 基因分型、拷貝數(shù)變異、恒溫和采用內(nèi)部陽性對(duì)照的 +/–Assay。為方便上述應(yīng)用領(lǐng)域，Life Technologies 提供了預(yù)先配制、已經(jīng)過質(zhì)量測(cè)試的即用型 TaqMan® Assay，可與 7500 系統(tǒng)配合使用?，F(xiàn)在您即可節(jié)省在分析優(yōu)化方面的精力。
升級(jí)至 7500 Fast 系統(tǒng)
您可以選擇升級(jí)至 7500 Fast 系統(tǒng)。該 7500 Fast 系統(tǒng)采用了我們的預(yù)混液配方，使您可以將實(shí)時(shí)熒光定量 PCR 程序縮短至 30 分鐘。
7500 系統(tǒng)包括：
? 可選擇 Dell® 商用系列筆記本或配有平板顯示器的臺(tái)式電腦
? 化學(xué)安裝試劑盒包括染料校正反應(yīng)板、RNA酶 P 儀器驗(yàn)證反應(yīng)板和應(yīng)用特異的 starter kit
? 儀器操作和分析軟件，以及 Primer Express®引物和探針設(shè)計(jì)軟件
? 包括入門指南在內(nèi)的豐富的文件集，適用于各種主要的實(shí)時(shí)熒光定量 PCR 應(yīng)用領(lǐng)域
? 由 Life Technologies 服務(wù)工程師提供的安裝服務(wù)
For Research Use Only. Not for use in diagnostic procedures.
7500實(shí)時(shí)熒光定量PCR系統(tǒng)規(guī)格參數(shù)：
| Format: | 96-well plate |
| Optics: | Tungsten-halogen lamp, 5 emission filters, CCD Camera, 5 excitation filters |
| Weight: | 75 lbs |
| Precision: | 99.7% confidence (5000 & 10000 copies) |
| Dimensions: | Width: 34 cm (13.39 in), Height: 49 cm (19.29 in), Depth: 45 cm (17.72 in) |
| Throughput: | 4 to 5-96-well plates per 8-hr day |
| Power Limit: | 950 W |
| Sensitivity: | 1 copy of RNase P gene (human genomic DNA) |
| Product Size: | 1 system |
| Dynamic Range: | 9 logs |
| Input Voltage: | 120V |
| Calibrated Dye: | SYBR® Green I, Cy3™, Cy5™, FAM™, JOE™, VIC™, Texas Red®, TAMRA™, ROX™, NED™ |
| Reaction Speed: | Standard |
| Sample Ramp Rate: | ± 1.1°C⁄sec |
| External Computer: | Notebook: Intel Core 2 Duo T5500 (1.66 GHz), 80 GB HD, 8X DVD, 1.0 G DDR2-533 MHz SDRAM |
| Peak Block Ramp Rate: | 2.5°C⁄sec |
| Temperature Accuracy: | 0.25°C (35°C to 95°C after 3 min) |
| Passive Reference Dye: | ROX (Separate Tube), No ROX, ROX (Pre-mixed) |
| Reaction Volume Range: | 20-100 µl |
| Software Compatibility: | Primer Express, 7500 System Software (RQ included), 21 CFR Part 11 Module (optional) |
| Temperature Uniformity: | 0.50°C (after 30 sec) |
| Thermal Cycling System: | Peltier-Based System |
| For Use With (Equipment): | 7500 System |
| Temperature Range (Metric): | 4-100°C |
| High Throughput Compatibility: | Multiplexing |
7500 實(shí)時(shí)熒光定量PCR系統(tǒng)訂購信息
| 產(chǎn)品名稱 | 訂貨編號(hào) | 數(shù)量單位 |
| 7500 Fast System Upgrade Kit | 4362143 | 1 臺(tái)儀器 |
| 7500 Real-Time PCR System with Dell™ Notebook | 4351104 | 1 臺(tái)儀器 |
| 7500 Real-Time PCR System with Dell™ Tower | 4351105 | 1 臺(tái)儀器 |
| Notebook Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems | 4359286 | 1 套 |
| Tower Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems | 4359284 | 1 套 |
| 7500 System SDS v1.4 21 CFR Part 11 Module | 4377354 | 1 套 |
7500熒光定量PCR儀訂購信息
| 產(chǎn)品描述 | 貨號(hào) | 數(shù)量 |
| 7500實(shí)時(shí)熒光定量PCR擴(kuò)增儀（配備筆記本電腦） | 4351104 | 1臺(tái) |
| 7500實(shí)時(shí)熒光定量PCR擴(kuò)增儀（配備臺(tái)式電腦） | 4351105 | 1臺(tái) |
| 7500實(shí)時(shí)熒光定量PCR擴(kuò)增儀快速模式升級(jí) | 4362143 | 1個(gè) |
| 7500實(shí)時(shí)熒光定量PCR擴(kuò)增儀SDS軟件21 CFR Part 11 模塊 | 4377354 | 1個(gè) |
7500Fast熒光定量PCR儀訂購信息
| 產(chǎn)品描述 | 貨號(hào) | 數(shù)量 |
| 新型高分辨率熔解曲線(HRM)軟件 | 4397808 | 1套 |
| 7500快速實(shí)時(shí)熒光定量PCR擴(kuò)增儀（配備筆記本電腦） | 4351106 | 1臺(tái) |
| 7500快速實(shí)時(shí)熒光定量PCR擴(kuò)增儀（配備臺(tái)式電腦） | 4351107 | 1臺(tái) |
| 7500實(shí)時(shí)熒光定量PCR擴(kuò)增儀快速模式升級(jí) | 4362143 | 1臺(tái) |
| 7500快速實(shí)時(shí)熒光定量PCR擴(kuò)增儀SDS軟件21 CFR Part 11 模塊 | 4377355 | 1套 |
